List of restriction enzyme cutting sites: A

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Main articles: List of restriction enzyme cutting sites and Restriction enzyme

Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site.

The following information is given for each enzyme:

  • Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature,[1][2] and bibliographical references. Note: When alphabetizing, enzymes are first ordered alphabetically by the acronyms (everything before the roman numeral); then enzymes of a given acronym are ordered alphabetically by the roman numeral, treating the numeral as a number and not a string of letters. This helps keep the entries ordered hierarchically while also alphabetic.(Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.[3][4]
  • REBASE Number: Number used to identify restriction enzymes in the REBASE restriction enzyme database. This database includes important information about the enzyme such as Recognition sequence, source, and Isoschizomers, as well as other data, such as the commercial suppliers of the enzyme.
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Displays the cut site and pattern and products of the cut. The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site.[5][6]
  • Isoschizomers and neoschizomers: An isoschizomer is a restriction enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8–10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None as of [date]" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists, as in this not listed enzyme:  Abc123I 

Whole list navigation[edit]

Restriction enzymes[edit]

A[edit]

Enzyme PDB code REBASE Number Source Recognition sequence Cut Isoschizomers
AaaI 1 Acetobacter aceti 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII
AagI 4 Achromobacter agile 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
AanI 15358 Arthrobacter aurescens RFL2 5' TTATAA
3' AATATT 		5' ---TTA   TAA--- 3'
3' ---AAT   ATT--- 5' 		PsiI, Csp1BORF7380P
AarI[7] 2892 Arthrobacter aurescens SS2-322 5' CACCTGC
3' GTGGACG 		5' ---CACCTGCN3N   NNNN--- 3'
3' ---GTGGACGN3NNNNN   --- 5' 		PaqCI
AasI 5465 Arthrobacter aurescens RFL3 5' GACN6GTC
3' CTGN6CAG 		5' ---GACNNNN   NNGTC--- 3'
3' ---CTGNN   NNNNCAG--- 5' 		DrdI, DseDI
AatI[8] 6 Acetobacter aceti 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AspMI, Eco147I,  Eco1524I,  PceI, Pme55I, SarI, Sru30DI, SteI
AatII[8][9][10][11] 7 Acetobacter aceti 5' GACGTC
3' CTGCAG 		5' ---GACGT   C--- 3'
3' ---C   TGCAG--- 5' 		Ssp5230I, ZraI
AauI 3010 Arthrobacter aurescens 5' TGTACA
3' ACATGT 		5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5' 		 BsmRI,  Bsp1407I, BsrGI, BstAUI, Ssp4800I, SspBI
AbaI 3029 Azospirillum brasilense UQ 1796 5' TGATCA
3' ACTAGT 		5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5' 		BclI, BsiQI, BspXII, BstT7I, FbaI, Ksp22I,  ParI 
AbeI 3097 Azotobacter beijerinckii Slo 54-028 5' CCTCAGC
3' GGAGTCG 		5' ---CC   TCAGC--- 3'
3' ---GGAGT   CG--- 5' 		BbvCI
AbrI 8 Azospirillum brasilense 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BluI, BspAAI, BssHI, EscI, PaeR7I, SciI, Sfr274I, Sol10179I, StrI, TliI
AbsI 14594 Arthrobacter sp. 7M06 5' CCTCGAGG
3' GGAGCTCC 		5' ---CC   TCGAGG--- 3'
3' ---GGAGCT   CC--- 5' 		None as of 4 Apr. 2019
AccI[12][13][14][15] 18 Acinetobacter calcoaceticus 5' GTMKAC
3' CAKMTG 		5' ---GT   MKAC--- 3'
3' ---CAKM   TG--- 5' 		FblI, XmiI
AccII[12][16] 19 Acinetobacter calcoaceticus 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		Bsh1236I, Bsp50I, BstFNI, BstUI, MvnI, SelI, BceBI, BepI, Bpu95I
AccIII[17] 20 Acinetobacter calcoaceticus 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		Aor13HI, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
Acc16I 2638 Acinetobacter calcoaceticus 16 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		AosI, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
Acc36I[18] 4162 Acinetobacter calcoaceticus 36 5' ACCTGC
3' TGGACG 		5' ---ACCTGCN3N   NNNN--- 3'
3' ---TGGACGN3NNNNN   --- 5' 		BfuAI, BspMI, BveI
Acc65I[19] 14 Acinetobacter calcoaceticus 65 5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		AhaB8I, Asp718I, KpnI, SthI
Acc113I 2665 Acinetobacter calcoaceticus 113 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5' 		AssI,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
AccB1I 15 Acinetobacter calcoaceticus B1 5' GGYRCC
3' CCRYGG 		5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5' 		BanI, BbvBI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I
AccB2I 2666 Acinetobacter calcoaceticus B2 5' RGCGCY
3' YCGCGR 		5' ---RGCGC   Y--- 3'
3' ---Y   CGCGR--- 5' 		 BfoI,  Bme142I, Bsp143II, BstH2I, HaeII, LpnI
AccB7I[20] 16 Acinetobacter calcoaceticus B7 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
AccBSI[21] 2733 Acinetobacter calcoaceticus BS 5' CCGCTC
3' GGCGAG 		5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5' 		BsrBI, BstD102I, Bst31NI, MbiI
AccEBI 17 Acinetobacter calcoaceticus EBF 65/65 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AsiI, BamHI, Bce751I, Bsp98I, BspAAIII, CelI, OkrAI, Uba4009I
AceI 2571 Anabaena cedrorum 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5' 		ApeKI, SuiI, Taq52I, TseI
AceII 2570 Anabaena cedrorum 5' GCTAGC
3' CGATCG 		5' ---GCTAG   C--- 3'
3' ---C   GATCG--- 5' 		AsuNHI, BmtI,  BspOI, , LlaG2I, NheI, PstNHI
AceIII 2569 Anabaena cedrorum 5' CAGCTC
3' GTCGAG 		5' ---CAGCTCN5N   NNNN--- 3'
3' ---GTCGAGN5NNNNN   --- 5' 		— None as of May 2010 —
AciI[11][22] 21 Arthrobacter citreus 5' CCGC
3' GGCG 		5' ---C   CGC--- 3'
3' ---GGC   G--- 5' 		SsiI
AclI[23] 2181 Acinetobacter calcoaceticus M4 5' AACGTT
3' TTGCAA 		5' ---AA   CGTT--- 3'
3' ---TTGC   AA--- 5' 		Psp1406I
AclNI 2667 Acinetobacter calcoaceticus N20 5' ACTAGT
3' TGATCA 		5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5' 		AhlI, BcuI, SpeI
AclWI 2668 Acinetobacter calcoaceticus W2131 5' GGATC
3' CCTAG 		5' ---GGATCN3N   N--- 3'
3' ---CCTAGN3NN   --- 5' 		AlwI, BinI,  BpuFI, BsrWI,  BspPI, BstH9I,  Bst31TI,  EacI,  Ral8I 
AcoI 11822 Acinetobacter calcoaceticus 5' YGGCCR
3' RCCGGY 		5' ---Y   GGCCR--- 3'
3' ---RCCGG   Y--- 5' 		EaeI, Bfi89I, CfrI, EcoHK31I
AcpI 22 Acidiphilium cryptum 25H 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		Asp10HI, Bpu14I, Bsp119I, BstBI, Csp45I, FspII, MlaI, NspV, SspRFI
AcpII 23 Acidiphilium cryptum 25H 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
AcrII 25 Anabaenopsis circularis 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AspAI, Bse64I, BseT9I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
AcsI[24] 2184 Arthrobacter citreus 310 5' RAATTY
3' YTTAAR 		5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5' 		ApoI,  CfaI,  FsiI, XapI
AcuI 5534 Acinetobacter calcoaceticus 5' CTGAAG
3' GACTTC 		5' ---CTGAAGN13NNN   --- 3'
3' ---GACTTCN13N   NN--- 5' 		BspKT5I, Eco57I
AcvI 5359 Aeromonas caviae 5' CACGTG
3' GTGCAC 		5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5' 		BbrPI, BcoAI, Eco72I, PmaCI, PmlI, PspCI
AcyI[25] 31 Anabaena cylindrica 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AhaII, AosII, BbiII, BsaHI, BstACI, Hin1I, HgiI, Hsp92I, Msp17I
AdeI 2991 Alcaligenes denitrificans Ss 3-028 5' CACNNNGTG
3' GTGNNNCAC 		5' ---CACNNN   GTG--- 3'
3' ---GTG   NNNCAC--- 5' 		BstIZ316I, DraIII
AeuI 33 Achromobacter eurydice 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AglI, AjnI, BseBI, BstNI, BstOI, Bst2UI, EcoRII, SleI, SspAI
AfaI[26] 37 Acidiphilium facilis 28H 5' GTAC
3' CATG 		5' ---GT   AC--- 3'
3' ---CA   TG--- 5' 		Csp6I, CviQI, CviRII, HpyBI, PabI, PlaAII, RsaI, RsaNI
Afa16RI 34 Acidiphilium sp. 16R 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa22MI, BspCI, EagBI, MvrI, NblI, Ple19I, PvuI, Psu161I, RshI, XorII
Afa22MI 35 Acidocella facilis 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa16RI, BspCI, EagBI, ErhB9I, NblI, Ple19I, Psu161I, PvuI, RshI
AfeI[27] 2669 Alcaligenes faecalis T2774 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AitI, Aor51H, Eco47III, FunI
AfiI 10489 Anoxybacillus flavithermus 5' CCN7GG
3' GGN7CC 		5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5' 		Bsc4I, BseLI, BslI, BflI, Bsc107I, BsiYI, Bst22I
AflI[28] Anabaena flos-aquae 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Asp745I, BamNI, BcuAI, CauI, Csp68KI, DsaIV, Eco47I, SinI
AflII[28][29][30] 39 Anabaena flos-aquae 5' CTTAAG
3' GAATTC 		5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5' 		BfrI, BspTI, Bst98I, BstAFI, BstPZ740I, Esp4I, MspCI, Vha464I
AflIII[28][10] 40 Anabaena flos-aquae 5' ACRYGT
3' TGYRCA 		5' ---A   CRYGT--- 3'
3' ---TGYRC   A--- 5' 		 Asp90I 
AgeI[11][31] 42 Agrobacterium gelatinovorum 5' ACCGGT
3' TGGCCA 		5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5' 		AsiAI, AsiGI, BshTI, CsiAI, CspAI, PinAI
AglI[32] 6652 Arthrobacter globiformis 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AeuI, Bse16I, Bse17I, Bse24I, BshGI, EcoRII, SleI, SniI, SslI
AgsI Agrococcus sp. 25 5' TTSAA
3' AASTT 		5' ---TTS   AA--- 3'
5' ---AA   STT--- 3' 		None as of 4 May 2019
AhaI 45 Alteromonas haloplanktis B8 5' CCSGG
3' GGSCC 		5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5' 		AseII, BcnI,  BpuMI,  CauII, Eco1831I, EcoHI, HgiS22I, NciI
AhaII Aphanothece halophytica 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AstWI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiHII, PamII
AhaIII[14][29][30][33] Aphanothece halophytica 5' TTTAAA
3' AAATTT 		5' ---TTT   AAA--- 3'
3' ---AAA   TTT--- 5' 		DraI, PauAII, SruI
AhaB8I Aphanothece halophytica B8 5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		Acc65I, Asp718I, KpnI, SthI
AhdI[34][35] Aeromonas hydrophila 5' GACN5GTC
3' CTGN5CAG 		5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5' 		AspEI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
AhlI 4838 Alteromonas haloplanktis SP 5' ACTAGT
3' TGATCA 		5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5' 		AclNI, BcuI, SpeI
AhyI 49 Aeromonas hydrophila 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		Cfr9I, CfrJ4I, EaeAI, EclRI, PaeBI, SmaI, TspMI, XmaI, XmaCI
AitI 54 Aquaspirillum itersonii 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AfeI, Aor51H, Eco47III, FunI
AjiI 10919 Acinetobacter johnsonii RFL47 5' CACGTC
3' GTGCAG 		5' ---CAC   GTC--- 3'
3' ---GTG   CAG--- 5' 		BmgBI, BtrI
AjnI 7185 Acinetobacter johnsonii R2 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AorI, ApaORI, ApyI, EcoRII, MvaI, Psp6I, PspGI, SleI, SspAI
AjoI 2604 Acinetobacter johnsonii 35 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, BloHII, BspMAI, CfrA4I, CfuII, PstI, SalPI, SflI, Srl5DI
AjuI 7740 Acinetobacter junii RFL46 5' GAAN7TTGG
3'CTTN7AACC 		5' ---NNNNN   NN6GAAN7TTGGN5NNNNNN   --- 3'
3' ---   NNNNNNN6CTTN7AACCN5N   NNNNN--- 5' 		None as of 28 Oct. 2020
AleI 5791 Aureobacterium liquefaciens 5' CACN4GTG
3' GTGN4CAC 		5' ---CACNN   NNGTG--- 3'
3' ---GTGNN   NNCAC--- 5' 		OliI
AlfI 6110 Acinetobacter lwoffii BH 32 5' GCAN6TGC
3' CGTN6ACG 		5' ---GCAN6TGCN9NNN   --- 3'
3' ---CGTN6ACGN9N   NN--- 5' 		None as of May 2010
AliI Acetobacter liquefaciens 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, BamHI, Bce751I, Bsp98I, BspAAIII, Nsp29132II, Pfl8I
AliAJI 59 Acetobacter liquefaciens AJ 2881 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, Asp713I, BspMAI, CflI, CstI, MhaAI, PstI, SalPI, Sst12I, YenI
AloI[36][37] 2812 Acinetobacter lwoffii Ks 4-8 5' GAACN6TCC
3' CTTGN6AGG 		5' ---GAACN6TCCN6NNNNNN   --- 3'
3' ---CTTGN6AGGN6N   NNNNN--- 5' 		None as of May 2010
AluI[12][14][38][39][40][41] 61 Arthrobacter luteus 5' AGCT
3' TCGA 		5' ---AG   CT--- 3'
3' ---TC   GA--- 5' 		AluBI, MltI
AluBI 16189 Arthrobacter luteus B 5' AGCT
3' TCGA 		5' ---AG   CT--- 3'
3' ---TC   GA--- 5' 		AluI, MltI
AlwI 65 Acinetobacter lwoffii 5' GGATC
3' CCTAG 		5' ---GGATCN3N   N--- 3'
3' ---CCTAGN3NN   --- 5' 		AclWI, BinI, BspPI,  BsrWI,  BstH9I,  BthII, Bth617I,  EacI
Alw21I Acinetobacter lwoffii RFL21 5' GWGCWC
3' CWCGWG 		5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5' 		AspHI, Bbv12I, Bsh45I, BsiHKAI, Bsm6I,  HgiAI, MspV281I
Alw26I[42] 63 Acinetobacter lwoffii RFL26 5' GTCTC
3' CAGAG 		5' ---GTCTCN   NNNN--- 3'
3' ---CAGAGNNNNN   --- 5' 		 BcoDI, BscQII,   BsmAI, BsoMAI,  BstMAI 
Alw44I Acinetobacter lwoffii RFL44 5' GTGCAC
3' CACGTG 		5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5' 		ApaLI, SnoI, VneI
AlwNI[43] Acinetobacter lwoffii N 5' CAGNNNCTG
3' GTCNNNGAC 		5' ---CAGNNN   CTG--- 3'
3' ---GTC   NNNGAC--- 5' 		CaiI
AlwXI 67 Acinetobacter lwoffii X 5' GCAGC
3' CGTCG 		5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5' 		BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
Ama87I Alteromonas macleodii 87 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, AvaI,  BmeT110I,  BsiHKCI, BsoBI, Eco88I, Nli3877I, NspSAI
AmaCSI 26714 Arthrospira maxima CS-328 5' GCTCCA
3' CGAGGT 		5' ---GCTCCAN8NNN   --- 3'
3' ---CGAGGTN8N   NN--- 5' 		None as of 4 May 2018
AocI 74 Anabaena sp. 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AyI, Bse21I, Bsu36I, Eco81I, Lmu60I, OaNI, MstII, SauI, SshAI
AocII Anabaena sp. 5' GDGCHC
3' CHCGDG 		5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5' 		BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
AorI 77 Acetobacter aceti orleanensis 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		BseBI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, Sth117I, Tai
Aor13HI[44] Acidiphilium organovorum 13H 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		AccIII, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
Aor51HI[45] 76 Acidiphilium organovorum 51H 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AfeI, AitI, Eco47III, FunI
AosI Anabaena oscillarioides 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
AosII Anabaena oscillarioides 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AstWI, BbiII, BsaHI, BstACI, Hin1I, HgiI, HgiGI, Msp17I, PamII
ApaI[11][30][46] Acetobacter pasteurianus pasteurianus 5' GGGCCC
3' CCCGGG 		5' ---GGGCC   C--- 3'
3' ---C   CCGGG--- 5' 		Bsp120I, PpeI, PspOMI
ApaBI[47] Acetobacter pasteurianus B 5' GCAN5TGC
3' CGTN5ACG 		5' ---GCANNNNN   TGC--- 3'
3' ---CGT   NNNNNACG--- 5' 		BstAPI
ApaCI Acetobacter pasteurianus C 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AliI, BamHI, BnaI, Bsp4009I, BstI, NspSAIV, Pfl8I, SolI, SurI
ApaLI[48][49] Acetobacter pasteurianus 5' GTGCAC
3' CACGTG 		5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5' 		Alw44I, SnoI, VneI
ApaORI 86 Acetobacter pasteurianus 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		AjnI, BstNI, BstOI, Bst1I, Bst2I, Fsp1604I, MvaI, Psp6I, PspGI
ApeKI[50] Aeropyrum pernix K1 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5' 		AceI, SuiI, Taq52I, TseI
ApiI 2315 Arthrobacter picolinophilus 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		Asp713I, Bsp63I, CfrA4I, PaePI, Pfl21I, Psp23I, PstI, SflI, Sst12I
ApoI[51] Arthrobacter protophormiae 5' RAATTY
3' YTTAAR 		5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5' 		AcsI, CfaI,  FsiI, XapI, EcoRI
AplI 25962 Arthrospira platensis NIES-39 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		See this site for a complete list.
ApyI 93 Arthrobacter pyridinolis 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		BciBII, BptI, BseBI, BstNI, BstOI, Bst2UI, Bst38I, Bst100I, MvaI
ApyPI 14756 Arcanobacterium pyogenes 5' ATCGAC
3' TAGCTG 		5' ---ATCGACN17NNN   --- 3'
3' ---TAGCTGN17N   NN--- 5' 		None as of 4 August 2019
AquI Agmenellum quadruplicatum PR-6 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		BcoI,  BmeT110I,  BsmAI, BsoMAI, BsoBI, BstSI, Eco27kI, OfoI
AquII 20238 Agmenellum quadruplicatum PR-6 5' GCCGNAC
3' CGGCNTG 		5' ---GCCGNACN17NNN   --- 3'
3' ---CGGCNTGN17N   NN--- 5' 		None as of 4 August 2019
AquIII 20239 Agmenellum quadruplicatum PR-6 5' GAGGAG
3' CTCCTC 		5' ---GAGGAGN17NNN   --- 3'
3' ---CTCCTCN17N   NN--- 5' 		BseRI
AquIV 20240 Agmenellum quadruplicatum PR-6 5' GRGGAAG
3' CYCCTTC 		5' ---GRGGAAGN16NNN   --- 3'
3' ---CYCCTTCN16N   NN--- 5' 		None as of 4/9/19
ArsI 19405 Arthrobacter sp. NTS 5' GACN6TTYG
3' CTGN6AARC 		5' ---NNNNN   NN7GACN6TTYGN5NNNNNN   --- 3'
3' ---   NNNNNNN7CTGN6AARCN5N   NNNNN--- 5' 		None as of Oct. 28 2020
AscI[11][52] Arthrobacter sp. 5' GGCGCGCC
3' CCGCGCGG 		5' ---GG   CGCGCC--- 3'
3' ---CCGCGC   GG--- 5' 		 PalAI, SgsI 
AseI[53] 96 Aquaspirillum serpens 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AsnI, BpoAI, PshBI, Sru4DI, VspI
AseII[53] 97 Aquaspirillum serpens 5' CCSGG
3' GGSCC 		5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5' 		AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Kpn49kII, Mgl14481I, NciI
AsiI Azotobacter sp. 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		ApaCI, BamHI, BnaI, Bsp4009I, BstI, OkrAI, Pfl8I, SurI, Uba4009I
AsiAI 2853 Arthrobacter sp. A7359 5' ACCGGT
3' TGGCCA 		5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5' 		AgeI, AsiGI, BshTI, CsiAI, CspAI, PinAI
AsiGI 10828 Arthrobacter sp. G 5' ACCGGT
3' TGGCCA 		5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5' 		AgeI, AsiAI, BshTI, CsiAI, CspAI, PinAI
AsiSI Arthrobacter sp. 5' GCGATCGC
3' CGCTAGCG 		5' ---GCGAT   CGC--- 3'
3' ---CGC   TAGCG--- 5' 		 RgaI, SfaAI, , SgfI
Asi256I 18472 Arthrobacter sp. Ck256 5' GATC
3' CTAG 		5' ---G   ATC--- 3'
3' ---CTA   G--- 5' 		See this site for a complete list.
Asi372I 15803 Arthrobacter sp. Mn372 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		Ppu10I, EcoT22I, Mph1103I, NsiI, Zsp2I, BfrBI, Csp68KIII, PinBI, SepI, SspD5II
AsnI[54] 98 Arthrobacter sp. N-CM 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AseI, BpoAI, PshBI, Sru4DI, VspI
AspI[55] Achromobacter sp. 699 5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AtsI, PflFI, PsyI, TelI, Tth111I
Asp10HI Acidiphilium sp. 10H 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BspT104I, BstBI, CbiI, Csp68KII, PlaII, PpaAI, Ssp1I
Asp10HII 100 Acidiphilium sp. 10H 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, AcpII, BasI, Esp1396I, PflBI, PflMI, Van91I
Asp26HI 111 Acidiphilium sp. 26H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, PctI
Asp27HI 112 Acidiphilium sp. 27H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp35HI, Asp36HI, Asp40HI,  BmaHI,  BsaMI, BscCI, BsmI
Asp35HI 117 Acidiphilium sp. 35H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp36HI, Asp40HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
Asp36HI 118 Acidiphilium sp. 36H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp40HI, Asp50HI,  BmaHI,  BscCI, BsmI, Mva1269I, PctI
Asp40HI 120 Acidiphilium sp. 40H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp27HI, Asp36HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
Asp50HI 122 Acidiphilium sp. 50H 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5' 		Asp26HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, Mva1269I
Asp700I[56] Achromobacter sp. 700 5' GAAN4TTC
3' CTTN4AAG 		5' ---GAANN   NNTTC--- 3'
3' ---CTTNN   NNAAG--- 5' 		BbvAI, MroXI, PdmI, XmnI
Asp713I 131 Achromobacter sp. 713 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AjoI, AliAJI, BloHII, CfrA4I, Ecl37kI, MhaAI, PstI, SflI, Srl5DI, YenI
Asp718I[57] Achromobacter sp. 718 5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		Acc65I, AhaB8I, KpnI, SthI
Asp745I Achromobacter sp. 745 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BsrAI, CauI, Eco47I, ErpI, FdiI, SinI, VpaK11AI, VpaK11BI
AspAI Alcaligenes sp. 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, Bse64I, BseT10I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
AspA2I 6959 Arthrobacter sp. A2 5' CCTAGG
3' GGATCC 		5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5' 		AvrBII, AvrII, BlnI, BspA2I, XmaJI
AspEI[58] Aureobacterium sp. 5' GACN5GTC
3' CTGN5CAG 		5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5' 		AhdI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
AspHI[59] Achromobacter sp. H 5' GWGCWC
3' CWCGWG 		5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5' 		Alw21I, Bbv12I, Bsh45I, BsiHKAI,  Bsm6I,  HgiAI, MspV281I
AspLEI Arthrobacter sp. LE3860 5' GCGC
3' CGCG 		5' ---GCG   C--- 3'
3' ---C   GCG--- 5' 		BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI
AspMI 2977 Acinetobacter sp. M 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI
AspMDI Alcaligenes sp. MD1 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BfuCI, Bsp143I, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
AspNI Anabaena sp. J3 5' GGNNCC
3' CCNNGG 		5' ---GGN   NCC--- 3'
3' ---CCN   NGG--- 5' 		 BmiI,  BscBI, BspLI, NlaIV, PspN4I
AspS9I Arthrobacter sp. S9 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AsuI, AvcI, Bac36I, Bal228I, FmuI, NspIV, PspPI, Sau96I
AssI 4930 Arthrobacter sp. 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5' 		Acc113I,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
AstWI Anabaena sp. 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiGI, Msp17I
AsuI[60] Anabaena subcylindrica 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AspS9I, AvcI, CcuI, Cfr13I, FmuI, MaeK81II, Nsp7121I, PspPI, UnbI
AsuII[61] Anabaena subcylindrica 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AcpI, Bpu14I, Bsp119I, BspT104I, Csp45I, FspII, LspI, NspV, SfuI
AsuIII Anabaena subcylindrica 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AsuIII, BbiII, BsaHI, BstACI, Hin1I, HgiHII, Hsp92I, Msp17I
AsuC2I 2901 Actinobacillus suis C2 5' CCSGG
3' GGSCC 		5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5' 		AhaI, AseII, BcnI, Eco1831I, EcoHI, Kpn49kII, Mgl14481I, NciI
AsuHPI[62] 2811 Actinobacillus suis HP 5' GGTGA
3' CCACT 		5' ---GGTGAN6NN   --- 3'
3' ---CCACTN6N   N--- 5' 		HphI, SspD5I
AsuNHI[62] Actinobacillus suis NH 5' GCTAGC
3' CGATCG 		5' ---G   CTAGC--- 3'
3' ---CGATC   G--- 5' 		AceII, BmtI,  BspOI,  LlaG2I, NheI, PstNHI
AtsI Aureobacterium testaceum 4842 5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AspI, PflFI, PsyI, TelI, Tth111I
AvaI[14][63][64] Anabaena variabilis 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, Ama87I, BsiHKCI, BsoBI, BspLU4I, Eco88I, NspIII, PlaAI
AvaII[12][14][41][63][64] Anabaena variabilis 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, Bme216I, BsrAI, Eco47I, Kzo49I, Psp03I, SmuEI, VpaK11AI
AvcI Actinomyces violaceoniger cristalomycini 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AspS9I, BshKI, BsiZI, Bsp1894I, BspBII, BspF4I, Bsu54I, Pde12I
AviII Anabaena variabillis (halle) 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AosI, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
AvrII[11][29] 173 Anabaena variabilis UW 5' CCTAGG
3' GGATCC 		5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5' 		AspA2I, AvrBII, BlnI, BspA2I, XmaJI
AvrBII 171 Arthrobacter variabilis 5' CCTAGG
3' GGATCC 		5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5' 		AspA2I, AvrII, BlnI, BspA2I, XmaJI
AxyI[65] 174 Acetobacter xylinus 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AocI, BliHKI, Bse21I, BspR7I, Bsu36I, CvnI, Eco81I, Lmu60I

§ An HF version of this enzyme is available

Notes[edit]

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