Axial twist theory

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Axial twist
Schema of the development of the axial twist. Developmental phases are (from top to bottom): (1) the embryo turns on its left side; (2) the anterior head grows in the same direction, but the rest of the body grows oppositely into a twist. So that ultimately (3) external bilateral symmetry is regained. Note, that there is no evolutionary pressure for internal symmetry so the heart (and other organs) remain asymmetric.
Details
Systemvertebrate body plan
Anatomical terminology

The axial twist theory (a.k.a. axial twist hypothesis) is a scientific theory put forward to explain a range of unusual aspects of the body plan of vertebrates (including humans).[1] It proposes that the rostral part of the head is "turned around" regarding the rest of the body.[2] This end-part consists of the face (eyes, nose, and mouth) as well as part of the brain (cerebrum and thalamus). According to the theory, the vertebrate body has a left-handed chirality.

The theory gives a phenomenal explanation and addresses how and when the twist between the end of the head and the rest of the body develops. It addresses the possible evolutionary history. The goal is to make testable predictions. For example, the theory predicted the aurofacial asymmetry, which was then found empirically.[3]

Explained phenomena include:

According to the axial twist developmental model, the anterior part of the head turns against the rest of the body, except for the inner organs. Due to this twist, the forebrain and face are turned around such that left and right, but also anterior and posterior are flipped in the adult vertebrate. There are some popular science videos and podcasts on the topic.[4][5]

History[edit]

The idea of a twist responded to severe inconsistencies in the prevailing scientific hypotheses to explain the contralateral organization of the forebrain (cerebrum and thalamus). Briefly, the visual map theory by Santiago Ramón y Cajal proposes that the optic chiasm restores the retinal image on the visual cortex,[6][7] but the loop of the optic radiation destroys this potential repair again, so there is no such consistency.[8] The parcellation theory proposes that an increasing brain size can conserve coincidental contralateral organization,[9] but does not explain the optic chiasm, nor that the pattern is conserved across all extant vertebrates regardless of brain size. Functional[10][11] or topological[12] explanations fail because same-side connections are just as important as crossing ones.[13] Moreover, these explanations leave open why the brain stem, including the cerebrum, has a same-side organization.

The idea of a twist was proposed two times independently. It does not suffer from these problems. The first presentation occurred at a conference in the 1970s by Marcel Kinsbourne,[14] but remained widely unknown. The first scientific publication in the form of a falsifiable scientific theory was made in 2012 by Marc de Lussanet and Jan Osse.[1] Kinsbourne subsequently also published his ideas in 2013.[2] In 2019, a novel kind of facial asymmetry, the aurofacial asymmetry, was predicted and confirmed on the basis of the axial twist theory.[3]

Kinsbourne developed his ideas from the dorsoventral inversion hypothesis by the early 19th-century naturalist Étienne Geoffroy Saint-Hilaire, proposing that the anterior head was the part of the head that was not inverted. He also stressed that the axial twist concerns all chordates. This proposal has the problem that such a turn does not occur during embryological development and has no evolutionary foundation.

De Lussanet and Osse used comparative functional morphology and embryonic development as a starting point to arrive at the axial twist hypothesis.

Embryology[edit]

Antero-dorsal view on the head and anterior trunk region of a zebrafish embryo (Danio rerio) on the egg surface. Source: figure 4 of reference [1]

Although the embryological development of the axial twist has not been studied explicitly, there are clear indications from the zebrafish and the chick.[1] The twist begins briefly after the neurulation and commences in a rostrocaudal (front-to-tail) direction.

Philipp Keller's group traced each cell of developing zebrafish embryos until the first heartbeat.[15] Tracing the movements of the cells in the future eye region and the hind part of the head, revealed opposite movement directions, in accordance with an axial twist. Whereas the left-eye eye-region cells tendentially moved outwards and downwards (ventrally), those of the right eye region moved out- and upwards, as visualized by a time-lapse video.[16] On the other hand, the surface cells of the hind side of the head moved to the left, consistent with an axial twist.[1]

The chick development has been studied well. The development is usually described according to the Hamburger–Hamilton stages.[17] The twisting begins during stage 6 on the rostral side of the head region[18] and commences until stage 14 towards the heart region.[19] Whereas the anterior head region rotates with the right side moving in an upward direction and the left side downward, the heart region moves in the opposite direction. In the end, the chick is turned on its right side, whereas the heart, not taking part in the twisting, has landed on the left side of the body.[1]

Genetic mechanisms[edit]

Main articles: Nodal signaling pathway and Left-right asymmetry

The axial twist emerges through opposite asymmetric development. This can be observed as a wave moving across the embryo from anterior to posterior. It is now well established, that the Nodal signaling cascade and the right-to-left flow produced by ciliated cells in the primitive streak are central in setting up the asymmetric organization. Three aspects of this growth wave are:[1]

Nodal, FGF8, and shh as well as the motor protein Kif5c have been associated with the asymmetric growth of the anterior primitive node, although only Nodal seems to be expressed before the initiation of the asymmetry[20][21]

The Nodal and cSnR genes control the asymmetric development of the heart.[22]

The right-side-turn of the body is tightly linked to the same genetic mechanisms.[22]

Developmental malformations[edit]

In holoprosencephaly, the hemispheres of the cerebrum (or part of it) are not aligned on the left and right sides but only on the frontal and occipital sides of the skull, and the head usually remains very small. According to the axial twist theory, this represents an extreme case of Yakovlevian torque,[23] and may occur when the cerebrum does not turn during early embryology.

Cephalopagus or janiceps twins are conjoined twins who are born with two faces, one on either side of the head. These twins have two brains and two spinal cords, but these are located on the left and the right side of the body.[24] According to the axial twist model, the two nervous systems could not turn due to the complex configuration of the body and therefore remained on either side.[1]

Evolution[edit]

The axial twist is thought to have evolved in a common ancestor of all vertebrates. Even the most distant clades of vertebrates — the agnathan lampreys and hagfish — possess an optic chiasm and contralateral brain organization,[25] as well as a left-sided heart and asymmetric bowels.[26] Also, every vertebrate has a contralateral organization of the forebrain.[25] Fossil skull impressions of early vertebrates from the Ordovician and later show the presence of an optic chiasm.[27]

Twisting and asymmetric development are well known from other deuterostomes — such as Hemichordata, Echinodermata, Cephalochordata, and Tunicata. Turning toward the side or upside-down also occurs frequently in these clades. For example, sea stars turn their mouth downwards, after the larva has briefly been attached to a substrate by a rudimentary stalk, with the mouth turned up. The adult lancelet buries itself with its back downward and their mouth reaching out. Some fish species tend to turn around when feeding from the water surface.

Thus, the axial twist evolved in an ancestor to all vertebrates, and possibly of all chordates or even in an early deuterostome.

Morphology[edit]

Caricature showing the external asymmetries due to the axial twist

The axial twist takes place in the early embryo of a vertebrate. There is an evolutionary pressure for animals towards bilateral symmetry, due to sexual selection (better looks to potential mates) and functional selection (e.g., better locomotion). The evolutionary pressure decreases with better symmetry. The pressure also decreases as a body part is less associated with the body surface and the locomotor system.

Consequently, the axial twist theory predicts that small, systematic asymmetries remain on the outside of the body and that these asymmetries are larger on the inside of the body.

Contralateral organization of the brain, Yakovlevian torque, and spinal asymmetry[edit]

Opposite rotational asymmetries as viewed from below. Left: the Yakovlevian torque in the cerebrum (exaggerated). Redrawn from Toga & Thompson.[28] Right: the opposite, rightward asymmetry of the thoracal spine.[29] Source: figure 4 of reference [3]
Main article: contralateral brain

The forebrain (cerebrum and thalamus) predominantly represents the opposite side of the body (and the visual world). However, motor control usually requires information from both sides of the body, and so the contralateral representation is by no means absolute. Rather, almost every region of the brain connects to both sides of the body.

The Yakovlevian torque[28] (a.k.a. "counterclockwise brain torque"[30] refers to an anatomical peculiarity of the normal brain. On average, the frontal lobes are asymmetric to the left (the right lobe appears slightly larger than the left), whereas the occipital lobe is asymmetric to the right; the central sulcus and temporal lobe of the right cortical hemisphere are further to the front than those on the left. Overall, these asymmetries are equivalent to a slight rotation of the cerebrum (i.e. torque). Such a rotation is exactly as predicted by the axial twist theory, given that the cerebrum is not a superficial structure.

The torque is also known as "occipital bending"[31] if it is more strongly expressed on the occipital side than on the frontal side.

The spine is slightly asymmetric. In healthy subjects, the thoracic vertebrae (vertebra T6-T12) were on average asymmetric, such that the mid-line points to the right (2.5° in T6).[29] Thus, the Yakovlevian torque and the spinal asymmetry are in opposite direction, just as predicted by the axial twist theory.

Decussations of the central nervous system[edit]

Some afferent decussations.
Pyramidal decussations.
Main article: decussation

If the right forebrain represents predominantly the left body and the left forebrain the right body, there should be positions of major nerve crossings behind the forebrain.

Anatomically, the contralateral organization of the forebrain is manifested by major decussations (based upon the Latin notation for ten, 'deca,' as an uppercase 'X') and chiasmas (after the Greek uppercase letter 'Χ,' chi). A decussation denotes a crossing of bundles of axonal fibers inside the central nervous system. As a result of such decussations: The efferent connections of the cerebrum to the basal ganglia, the cerebellum, and the spinal cord are crossed; and the afferent connections from the spine, the cerebellum, and the pons to the thalamus are crossed.[25] Thus, motor, somatosensory, auditory, and visual primary regions in the forebrain predominantly represent the contralateral side of the body.

Most afferent and efferent connections of the forebrain have bilateral components, especially outside the primary sensory and motor regions. As a result, hemiplegia that is acquired in early childhood can sometimes be completely compensated over time.

The visual sensory and motor system[edit]

Main articles: Visual system and optic chiasm

Four of the cranial nerves serve the eye directly: one sensory and three motor nerves. The optic nerve is sensory and crosses the midline in the optic chiasm. The oculomotor nerve, trochlear nerve, and abducens nerve are motor nerves that control one or more of the eye muscles. The oculomotor nerve crosses the midline before leaving the central nervous system. The trochlear nerve crosses the midline in a chiasma on the dorsal side and the abducens innervates an eye muscle on the same side.[32]: Fig. 17.8 

In the light of the axial twist theory, this complicated pattern can be understood. The eyes, like the mouth and the nose originate from the anterior head region, i.e. in front of the twist. The only cranial nerve that originates from the forebrain is the olfactory nerve. All other cranial nerves originate from regions of the central nervous system that lie behind the twist.[1]

The optic nerve inserts on the optic tectum of the midbrain. In tetrapods and bony fish it also branches off to the LGN of the thalamus in the forebrain, but not in other vertebrates such as sharks and skates). In sharks, the visual center in the cerebrum obtains its fibers from the optic tectum. On the way, these fibers cross the midline again so that each hemisphere of the cerebrum of sharks represents the eye on the same side.[33][34] Thus, the optic tract largely follows the prediction of the axial twist theory. The branch towards the LGN has been acquired later in the evolution and thus makes an exception.

The oculomotor and trochlear nerves originate from the midbrain and cross the midline as predicted by the theory.

The abducens nucleus is located in the pons. The abducens nerve innervates the lateral rectus muscle of the eye in most vertebrates, except lampreys and hagfishes. It thus seems that the lateral rectus muscle evolved later and independently of the other eye muscles, and presents an exception to the axial twist model.

The olfactory system[edit]

The olfactory (smelling) tracts do not chiasmate

The olfactory tracts run parallel to the optic tract but do not form a chiasm. Accordingly, each olfactory bulb connects to the same-side centers of the frontal cerebrum. This is entirely consistent with the axial twist theory because the nose is part of the anterior head region which twists along with the forebrain. Since the primary olfactory centers are at home in the cerebrum (olfaction is the only sense that originates in the cerebrum), each olfactory lobe is predicted to be represented by the cerebrum on the same side.

Aurofacial asymmetry[edit]

Exaggerated schema of the aurofacial asymmetry as predicted by the axial twist theory. During embryology and development, the face elements (red) are predicted to move toward the center from the left, with respect to the mid-plane between the ears. Source: figure 1c of reference[3]
Main article: facial symmetry § aurofacial asymmetry

The aurofacial asymmetry expresses the position of the face (eyes, nose, mouth) with respect to the plane perpendicular to the axis through the ears. As shown in the graph, the asymmetry decreases until the age of 13. Since the axial twist is located between the ears and the face, it is predicted that the face grows from the left to the midline, as is indeed the case.[3]

Left heart and stomach, right liver, asymmetric bowels[edit]

Diagram of the human stomach, intestines, and rectum.
Main article: Heart development

The inner organs of the trunk are the regions on the body that are least mechanically associated with locomotion and the external body, and so are predicted by the axial twist theory to be the most asymmetric regions of the body. Other bilaterally symmetric animals such as insects and annelids are bilaterally symmetric also on the inside. The asymmetric development of the heart is well-researched.[22][35]

The question of why the heart should have a left-sided orientation is frequently asked,[36] and has been encountered with another question such as “why should it be symmetric” or a proposal that it might provide more efficient usage of space inside the body. The axial twist theory is presently[when?] the only scientific explanation.

The asymmetric orientation and position of the gastrointestinal tract, the liver, and the pancreas have been studied much less than the heart, probably because the heart is very prominent already in the early embryonic stages and everyone is aware of their heart throughout life.

Relation to other theories[edit]

See also: Contralateral brain § Theories

There are no other theories or hypotheses that explain the entire spectrum covered by the axial twist theory. Several hypotheses have been proposed to explain (certain aspects of) the contralateral brain (i.e. the left forebrain representing mostly the right body and the right forebrain representing mostly the left body).

No hypotheses have been published for the asymmetry of the spine. There is no other theory to explain the left position of the heart or the asymmetric orientation of the bowels and related organs.

The Yakovlevian torque is often thought to reflect the lateralization of specific functions in the brain.[28]

Inversion hypothesis[edit]

Main article: Inversion (evolutionary biology)

In 1822 the French zoologist Étienne Geoffroy Saint-Hilaire noted that the organization of dorsal and ventral structures in the crayfish (an arthropod) is opposite that of mammals, and he proposed that mammals and other vertebrates are turned upside down.[37][38] As explained above, Marcel Kinsbourne proposed that the body (soma) but not the anterior head is inverted (hence somatic twist hypothesis).[2]

There is molecular evidence for the inversion hypothesis in almost all groups of deuterostomes.[39][40]

Open questions[edit]

The axial twist theory is a novel scientific discipline and very few scientific papers have presently addressed it directly.[1][2][8][3] Although a considerable volume of research exists on the genetic and embryological mechanisms of asymmetric development, an open question is how the twist is initiated and how the inversion of the left-right and up-down axes in the anterior head region is established.

The embryology of the twisting has been addressed only rudimentary in the chick and the zebrafish.[1] The differences in timing and mechanisms across the vertebrate clades are completely unknown.

The evolution of the axial twist is an open question. The founders of de axial twist idea (de Lussanet & Osse, and Kinsbourne) agree that the axial twist is universal in vertebrates and probably is a feature of all chordates.[1][2] Although the asymmetric development of other chordates such as the Lancelet has been studied in detail, no study has analysed this development in the light of the axial twist theory. Moreover, even other deuterostomes, i.e. the echinoderms (sea stars, sea lilies, etc.) show a marked asymmetric development and even an axial twist[41] This twist has remarkable similarities to that in vertebrates, but no study has addressed this at present. Lastly, the asymmetric and twisted development is well known from gastropods and the relation to asymmetric development in vertebrates is an important question.

It has been proposed that problems in the axial twist development may play a central role in developmental malformations such as holoprosencephaly[1] and skoliosis[3] but these have not been looked into.

See also[edit]

References[edit]

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